Imperial character story modes:
Complete the game in the Imperial storyline.


Shinsengumi character Story modes:
Complete the game in the Shinsengumi storyline.

All story modes:
Complete the game in both storylines.

Harada Sanosuke:
Complete Chapter 5 to unlock Harada Sanosuke in two
player mode.

Hijikata Toshizo:
Complete Hijikata Toshizo's story mode quest to unlock
him in two player mode.

Hirayama Gorou:
Kill Hirayama Gorou at the end of Chapter 1 to unlock
him in two player mode.

Itou Kashiitarou:Kill Itou Kashiitarou at the end of Chapter 4 to unlock
him two player mode.

Katsura Kogoro:
Complete Katsura Kogoro's story mode quest to unlock
him in two player mode.

Kondo Isami:
Complete Kondo Isami's story mode quest to unlock him
in two player mode.

Nagakura Shinpachi:
Complete Chapter 5 to unlock him in two player mode.

Okita Souji:
Complete Okita Souji's story mode quest to unlock him
in two player mode.

Saigo Takamori:
Complete Saigo Takamori's story mode quest to unlock
him in two player mode.

Saito Hajime:
Complete Saito Hajime repeatedly on Te-awase to unlock
him in two player mode.

Sakamato Ryoma:
Complete Sakamato Ryoma's story mode quest to unlock
him in two player mode.

Serizawa Kamo:
Complete Serizawa Kamo's side quest to unlock him in
two player mode.

Shinsengumi Lower Class member:
Start a game on the Shinsengumi storyline to unlock
this character in two player mode.

Takeda Kanryuusai:
Complete the mission with Takeda Kanryuusai at Masuya
to unlock him in two player mode.

Tetsunosuke Ichimura:
Meet Tetsunosuke Ichimura during Chapter 6 of the Shinsengumi
storyline to unlock in two player mode.

Toudou Heisuke:
Kill Toudou Heisuke at the end of Chapter 4 to unlock
him in two player mode.

Two Hitokiri:
Complete the story mode for Two Hitokiri to unlock
him in two player mode.

Yamanami Keisuke:
Kill Yamanami Keisuke at the end of Chapter 3 to unlock
him in two player mode.

Yamazaki Susumu:
Start a game in the Shinsengumi storyline to unlock
him in two player mode.

School power-up items:

Choshu Ryu: In the Imperial storyline, get all techniques of the
school then defeat the sword master.


Edo Ryu: In the Imperial storyline, get all techniques of the
school then defeat the sword master.


Hokushin Ittou Ryu: In either storyline, get all techniques of
the school then defeat the sword master.


Satsuma Ryu (Jigen Ryu): In the Imperial storyline, get all techniques
of the school then defeat the sword master.


Shintou Munen Ryu: In either storyline, get all techniques of
the school then defeat the sword master.


Spear Ryu (Houzouin Ryu): In the Shinsengumi storyline, get all
techniques of the school then defeat the spear master.


Tama Ryu (Tennen Rishin Ryu): In the Shinsengumi storyline, get
all techniques of the school then defeat the sword master before
Chapter 6.